| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Colonization of polystyrene microparticles by Vibrio crassostreae: light and electron microscopic investigation
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| Autores: |
Foulon, Valentin
Le Roux, Frederique
Lambert, Christophe
Huvet, Arnaud
Soudant, Philippe
Paul-pont, Ika
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| Data: |
2016-10
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| Ano: |
2016
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| Resumo: |
Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures. A longer bacterial attachment (6 days) was observed on irregular micro-PS compared to smooth particles (<10h) but complete decolonisation of all particles eventually occurred. The presence of natural marine agreggates around micro-PS led to substantial and perennial colonisation featuring monospecific biofilms at the surface of the aggregates. These exploratory results suggest that V. crassostreae may be a secondary coloniser of micro-PS, requiring a multi-species community to form a durable adhesion phenotype. Temporal assessment of microbial colonisation on microplastics at sea using imaging and omics approaches are further indicated to better understand the microplastics colonisation dynamics and species assemblages.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00351/46210/45908.pdf
DOI:10.1021/acs.est.6b02720
https://archimer.ifremer.fr/doc/00351/46210/
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| Editor: |
Amer Chemical Soc
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| Formato: |
application/pdf
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| Fonte: |
Environmental Science & Technology (0013-936X) (Amer Chemical Soc), 2016-10 , Vol. 50 , N. 20 , P. 10988-10996
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| Direitos: |
2016 American Chemical Society
info:eu-repo/semantics/openAccess
restricted use
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